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The Method
The Analysis
Demonstration Tests

 

         Two sets of typical test results are reported. Both sets of analyses were performed "blind" (the operator did not know the origin of the bones). The purpose of the tests was to correctly identify bone fragments as either "human" or "animal" using pRIA. In each test, the reactivity of the antisera was first verified. The tables with the blue backgrounds show these results. Note the highest numbers in red (the greatest percentage reaction) correspond to the correct result. (i.e. in Test 1 the known human sample gave the highest value to the human antisera, the known elephant sample gave the highest value with to the elephant antisera and the bison gave the highest value to the bison antisera). Multiple animal antisera were used to add certainty to a "non-animal" identification. The unknowns were simultaneously tested with the known samples and results derived.

TEST 1
1. Six bone samples were tested. Three were fossil human and three were fossil animal (mastadon and mammoth).
2. The samples were split into pairs, so that there were 12 total samples to be measured (two from each of the six samples).
3. The analyst did not know what the bones were. A list of candidates was provided with the samples. The list only said they were either human, elephant, or bison.
(pRIA correctly identifies the presence of the "pairs" and each specimen as "human" or "animal".)

TEST 2
1. One bone sample, a modern calf bone, was analyzed to test the reliability of "human vs. animal" identification when the animal specimen is "fresh" and highly preserved. The test was also designed to demonstrate the ability of pRIA to further identify to Family (e.g. Bovidae, Cervidae, etc.).
2. A list of candidates was provided with the sample. The list said it was either human, calf, pig, deer, or chicken.
3. The analyst did not know what the bone was. (pRIA correctly identifies the specimen as "animal", and correctly identifies it as a member of the Bovidae family.)